TOOL BOX
Databank
Visualization/Drawing
Analyzing NGS Data
Next-Generation Transcriptome Assembly
Sequence Alignment
Analyzing Sequence Data
SAMtools (SAMtools, BCFtools and HTSlib)
Tools for high-throughput sequencing data
Seurat
An R toolkit for single cell genomics
Trimmomatic
Trimming illumina paired-end and single ended data
Nanopolish(for nanopore sequencing data)
Signal-level algorithms for MinION data
Bowtie2
A tool for aligning sequencing reads to reference
BWA
Burrow-Wheeler Aligner for short-read alignment
CheckM
Assess the quality of microbial genomes recovered from isolates, single cells, and metagenome
Porechop
Finding and removing adapters from Nanopore reads
Rerio
Research release basecalling models and configurations
Bonito
A PyTorch Basecaller for Oxford Nanopore Reads
Galaxy
Galaxy is a use-friendly web interface to a lot of bioinformatics tools. It is very popular for analyzing NGS data.